It has long been known that phagocyte has functions of phagocytosis and immune reaction in human. The structures and functions of alveolar, peritoneal and spleen macrophages have extensively been studied. And the recent observations centered on more detailed study for kinds, localization, origin and contents of the macrophage enzymes.
Many investigators have reported that during phagocytosis, acid phosphatase synthesized in the lysosome exhibited the highest activity among the macrophage enzyme. Most of investigators have also insisted that the macrophage exhibited higher acid phosphatase activity when toxic materials or antigens were administered to animals.
The present study was undertaken in order to observe the migration of macrophage and acid phosphatase activity of macrophage in white pulp of the spleen of albino rats treated with cycloheximide.
In this experiment, healthy Wistar strain albino rats which at age of 30-40 days weighing about 100gm were divided into control and cycloheximide treatment groups; each group was subdivided into 6, 12 and 24 hours groups, respectively.
The animals of the cycloheximide treatment group were administered 5 mg per kg of body weight of cycloheximide dissolved in physiological saline solution by intraperitoneal injection.
After sacrificing experimental animals, paraffin ribbon was prepared for hematoxylin and eosin stain and freezing ribbon for the detection of acid phosphatase activity by the Gomori method. The preparations were observed under the light microscope.
The results obtained were as follows:
It was observed that the number of macrophage migrated and the acid phosphatase activity of the macrophage were gradually increased in marginal zone, marginal sinus and germinal center on the white pulp in the spleen with time after the administrations of cycloheximide in albino rats.
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